PCL20-117: Can Green Tea Extract Inhibit the Tumor Growth and Invasiveness of 5-FU and Gemcitabine-Resistant Metastatic and Remetastatic Cell Lines in a Hamster Pancreatic Cancer Model?

View More View Less
  • a University of Sao Paulo, Hospital Sirio Libanes, Sao Paulo, Brazil
  • b Advantage Health, Sao Paulo, Brazil
  • c Toyama University, Toyama, Japan
  • d UNAERP, Sao Paulo, Brazil
  • e Faculdade de Medicina de Olinda, Brazil

Green tea contains substances called polyphenolspolyphenols, which contributes to its anticancer activity. They may interfere in several processes involved in cell replication, causing tumor cell death. To study whether the same drugs, which could inhibit the tumor growth in the parental pancreatic cancer cell line, could inhibit in the metastatic and remetastatic pancreatic cancer ones, comparing the inhibition with green tea extract. METHODS: HaP-T1:a cell line derived from nitrosamine induced pancreatic cancer,MS-PaS-1:a pancreatic metastatic cell line established from a “return trip” metastases of liver implanted tumor, which showed pancreatic metastases, and MS-PaS-2:a pancreatic remetastatic cell line established from metastases of MS-PaS-1 were used for the experiments. 5-Fluorouracil (5FU), Gemcitabine (GEM) and green tea extract (GTE) were used. MTT assay and MTT agarose assay were performed. In vitro chemoinvasion assay was done. RESULTS: The inhibitory concentration (IC50) of 5-FU,which inhibited the HaP-T1,had to be increased in 50 folds to inhibit MS-PaS-1,and 100 folds to inhibit MS-PaS-2.GEM had to be increased 5 folds to inhibit MS- PaS-1,and 25 folds to inhibit MS-PaS-2.However, IC50 of GTE had to be increased 3 folds to inhibit MS-PaS-1,and 5 folds to inhibit MS-PaS-2.GTE inhibited the invasiveness of 3 cells lines in a dose dependent manner. CONCLUSIONS: GTE may be a new cancer strategy for pancreatic cancer because it could inhibit the tumor growth and invasiveness in metastatic and remetastatic cell lines as well as in primary tumor cells in small doses when compared to 5-FU and GEM, leading to the fact that side effects could be decreased. However, further studies will be necessary to clarify.

If the inline PDF is not rendering correctly, you can download the PDF file here.

Corresponding Author: Cintia Yoko Morioka, MD, PhD